Optimization of Extraction Process of Active Components from Ganoderma lucidum by Low-temperature Continuous Phase Transition
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Abstract:
In this study, the bioactive components of Ganoderma lucidum were extracted by low-temperature continuous phase transition extraction technology. The yields of the extracted solids, total sugars and total triterpenes from Ganoderma lucidum were taken as the main indices to study the effects of factors such as extraction temperature, extraction time, extraction solvent and solvent concentration on the bioactive components extracted by low-temperature continuous phase transition from Ganoderma lucidum. The extraction conditions for the bioactive components in Ganoderma lucidum were optimized by orthogonal experiments, and the molecular weight distribution of the total polysaccharides in the water extract obtained under such optimal conditions was analyzed by GPC (gel penetration chromatography). The experimental results showed that the optimal conditions for low-temperature continuous phase transition extraction of bioactive components from Ganoderma lucidum were: water extraction temperature, 100 ℃; desorption l temperature, 78 ℃; water extraction solvent flow rate, 30 L/h; total extraction time, 5 h; Ganoderma lucidum residues after water extraction were extracted using alcohol; ethanol extraction temperature, 85 ℃; desorption temperature, 68 ℃; ethanol extraction solvent flow rate, 28 L/h; total extraction time, 4 h. The yields of total solids, total sugars and total triterpenes extracted by low-temperature continuous phase transition from Ganoderma lucidum were 7.56 g/100 g, 1.14 g/100 g and 1.17 g/100 g, respectively, which were 15.60%, 23.91% and 333.33%, respectively, higher than those by traditional water extraction. According to the GPC spectrum of the water extract, it was found that the low-temperature continuous phase transition extraction led to two more components than the traditional process. The yield and number of the bioactive components extracted by the low-temperature continuous phase transition extraction process were significantly increased compared to the traditional process, which is suitable for large-scale industrial production. This study provides certain theoretical support for deep processing of Ganoderma lucidum.
