Ultra-high-pressure Pretreatment Reduces Biogenic Amines in Fermented Ruditapes philippinarum Sauce While Improving Its Quality
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Abstract:
In this study, Ruditapes philippinarum with/without a pretreatment by ultra-high-pressure sterilization or heat sterilization was hydrolyzed preliminarily with protease and then rapidly fermented with Pediococcus pentosus at 35 ℃ for 36 h. The results showed that during the fermentation process, the total acid content of the fermented Ruditapes philippinarum sauce increased gradually, with its pH value decreasing gradually and the amino acid nitrogen content increasing gradually. The content of amino acid nitrogen of the heat pretreated group increased slowly. At the 36th h, the content of amino acid nitrogen of the control group, ultra-high-pressure pretreated group and heat pretreated group was 0.68, 0.68 and 0.42 g/100 mL. The contents of total volatile basic nitrogen (TVB-N), putrescine and cadaverine in the fermented Ruditapes philippinarum sauce could be reduced by ultra-high-pressure pretreatment. At the 36th h, the contents of TVB-N of the control group, ultra-high-pressure pretreated group and heat pretreated group were 79.43, 65.15 and 43.73 g/100 mL, with their cadaverine contents as 140.96, 0.83 and 0.79 mg/L, respectively. The putrescine content of the control group was 0.64 mg/L, whilst those of the ultra-high-pressure pretreated group and the heat pretreated group were lower than the detection limit. Compared with the heat pretreated group, the ultra-high-pressure pretreated group could make the special flavor of the fermented Ruditapes philippinarum sauce unchanged. The total sensory evaluation scores of the control group, ultra-high-pressure pretreated group and heat pretreated group were 13.20, 18.20 and 15.60, respectively. Therefore, ultra-high-pressure sterilization can be used as a pretreatment for Ruditapes philippinarum fermentation. This approach can reduce the contents of TVB-N, putrescine and cadaverine, improve the sensory score and ensure the safety of the fermented Ruditapes philippinarum sauce. Compared with the heat pretreatment, ultra-high-pressure pretreatment can ensure that the original special flavor and fermentation outcome of the fermented sauce remained unchanged.
