Transcriptomic Analysis of the Effects of Ultrasound Induction on Peanut Sprout and the Mining of Genes Involved in Phenylpropanoid Biosynthesis
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Abstract:
To analyze the differences in genetic and metabolic pathways during germination in peanuts, peanut varieties were pre-screened to identify those with resveratrol enrichment for further evaluation. Germinated peanuts subjected to induction with ultrasound (CS) were used as the experimental group, and uninduced germinated peanuts (KB) were used as the control group. A total of 1104 differentially expressed genes (DEGs) was obtained, including 521 upregulated genes and 583 downregulated genes. In the Gene Ontology enrichment analysis of the DEGs, functional analysis of the top 20 categories with obvious enrichment levels was performed. Twenty-one different cluster of orthologous group functional annotations were obtained, which primarily involved carbohydrate transportation and metabolism, post-translational modification, and protein conversion. There were 21 biosynthetic pathways in which DEGs were enriched in phenylpropanoids, which are the most significant pathways for Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. The KEGG database showed that the genes arahy.Tifrunner.gnm1.ann1.DXZI51, arahy.Tifrunner.gnm1.ann1.VGN2GE, and arahy.Tifrunner.gnm1.ann1.Y23DM6 are involved in phenylpropanoid biosynthesis. These results indicate that ultrasound induction can regulate the expression of genes related to phenylpropanoid biosynthetic pathways in germinating peanuts, and transcriptomic changes can be determined at the molecular level in peanut buds induced by ultrasound.
