Antioxidant Effect of Peptides of Wild Apricot from Yiwu County in Xinjiang in Aging Mouse Model
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Abstract:
In this study, enzymatic hydrolysis was used to extract the peptides in wild apricot from Yiwu County of Xinjiang, and its antioxidant effect was examined in aging mouse model. Through single factor experiments, the effects of enzyme-substrate ratio, material-to-liquid ratio, extraction time, pH and enzymatic hydrolysis temperature on hydrolysis degree were examined, and the extraction process for the wild apricot peptide was optimized through combining single factor and orthogonal experiments. Taking the extract as the raw material, D-galactose was used to establish an aging mouse model, and the mice were divided into the blank control group, the low-, medium-, and high-dose wild apricot peptide groups (50, 75 or 100 mg/kg), the aging model group and the Vc positive control group. After 30 days of continuous intragastric administration, the GSH-Px activity, SOD activity and MDA content in the serum, liver tissue and brain tissue homogenates were determined. The optimal conditions for extracting wild apricot peptides by enzymatic hydrolysis were as follows: enzyme-substrate ratio, 4%; enzymolysis time, 120 min; pH, 8; enzymolysis temperature, 65 ℃; material-liquid ratio, 1:25. The hydrolysis degree and peptide yield were 35.73 % and 7.22%, respectively. Compared with the model group, the medium-dose and high-dose peptide groups could significantly increase the activities of T-SOD and GSH-Px in mouse serum, liver tissue and brain tissue (p<0.05), and reduce the content of MDA (p<0.05). Among which, the serum contents of serum T-SOD, MDA and GSH-Px were 177.24 U/mL, 9.05 nmol/mL and 261.59 U/mL. Respectively, in high dose groups, were similar to those of the blank control group. The results showed that the peptides from the wild apricot in Yiwu County of Xinjiang exhibited good anti-oxidant effects in the aging mouse model.
