Protective Effect of Flavonoids from Young Fruit of Golden Pomelo on Oxidative Damage of Erythrocyte Induced by AAPH
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Abstract:
In this study, microwave-assisted ultrasonic were used to extracted flavonoids from golden pomelo, after purified by AB-8 macroporous resin and preparative liquid chromatography, the components of PF were identified by liquid chromatography-mass spectrometry (LC-MS). The results showed that PF contained 35 components, among which the contents of cascaroside A, stachydrine, 2-O-caffeinated arbutin and betaine were 15.57%, 1.6%, 1.15% and 0.13%, respectively. In the model of erythrocyte hemolysis induced by AAPH, the hemolysis rate of the experimental group was 31.63% after high concentration treatment of PF, which was significantly lower than that of the AAPH group (52.79%). In the enzymatic antioxidant system, the enzyme activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), after high concentration treatment, were 241.04 U/L, 93.29 U/L and 72.93 U/L, respectively, which were lower than those in AAPH group. In the non-enzymatic antioxidant system, the contents of glutathione (GSH) and malondialdehyde (MDA) after high concentration of PF treatment were 1233.94 μmol/L, 27.39 nmol/L, respectively, and the activity of lactate dehydrogenase (LDH) was 314.75 U/L, and all showed a does response. The result shows that PF can inhibit AAPH induced oxidative stress mostly by scavenging free radicals through non-enzymatic system, which provides a certain research basis for the development of natural products of young pomelo.
