Optimization of the Extraction Process of Stilbene Compounds and Their Tyrosinase Inhibitory Activity
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Abstract:
In this study, the enrichment and extraction processes of three stilbene compounds (oxyresveratrol, resveratrol and resveratrol glycosides) from the mulberry root were investigated, and the antioxidant activity and tyrosinase inhibition of the extracts were also studied. The results showed that the optimal conditions for enriching stilbene compounds were determined by single factor test and response surface tests as follows: extraction time, 1 h; solid-liquid ratio, 1:25; ethanol concentration, 90%. Under these conditions, the contents of oxyresveratrol, resveratrol and resveratrol glycosides in mulberry root were 2923.59 μg/g, 32.45 μg/g and 3.15 μg/g, respectively, and the yield of the extract rich in three stilbene compounds was 10.23%. In addition, the extract showed a strong DPPH free radical scavenging ability (IC50=0.063 mg/mL) and ABTS free radical scavenging capacity (IC50=0.008 mg/mL). Furthermore, the extract showed a stronger tyrosinase inhibitory activity than arbutin, with its tyrosinase inhibitory rate IC50 (2.514 μg/mL) being only 16% of that of arbutin (IC50=15.551 μg/mL). In summary, the highly active stilbene extract has the potential as a natural antioxidant and whitening agent, and has great application value in the fields of food, beauty and medicine. Mulberry root can be used as an important natural source of stilbene compounds, which is worth to be developed and utilized as a raw material for anti-oxidation and whitening.
