Optimization of Solid Fermentation Conditions for Cordyceps militaris with Low GI, High Content of Cordycepin and Pinastatin
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Abstract:
In order to obtain the fermentation substance of Cordyceps militaris with high active components and low glycemic index (GI), the solid fermentation medium formula was optimized by single factor and orthogonal test, and the optimal culture time was determined using the expected glycemic index (eGI), cordycepin and pinastatin content of fermentation medium as evaluation indexes. The results showed that the optimal formulation of C. militaris solid fermentation medium consisted of 70% main materials and 30% auxiliary materials. The main materials were composed of rice and oat with mass ratio of 4:6, and the auxiliary materials were soybean meal. After mixing, a liquid complete medium containing 10 g/L glycine was added at the ratio of material to liquid (m:V) of 1:0.9, and the mycelia was cultured at 25 ℃ in dark for 18 days. Under these conditions, the eGI value of fermentation substance was 53.86, which decreased by 9.39% compared with the initial formula and reached the low GI level. The contents of cordycepin and pinastatin were 12204.55 mg/kg and 1021.48 mg/kg, respectively, which were increased by 348.13% and 81.79% compared with the initial formula. The optimized fermentation substance of C. militaris with low GI value, high cordycepin and pinastatin content provided raw materials for the development of functional food with low GI value.
