Rapid Identification of Authentic Donkey Skin Based on Gas Chromatography
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Abstract:
In order to achieve more comprehensive control of the quality of E-jiao, a rapid method based on gas chromatography for identifying authentic donkey skin was established. After the animal sebum was extracted and methylated, fingerprints of total fatty acids in donkey skin, sheep skin, and cow skin were preliminarily established. The relative contents of myristic acid and palmitoleic acid in donkey, sheep and cow hides were determined by normalization method, and quantitative analysis of myristic acid and palmitoleic acid in donkey, sheep and cow hides was carried out by the internal standard method (using methyl heptadecanoate as the internal standard). The results showed high differences in myristic acid and palmitoleic acid among donkey, sheep and cow hides. The relative content of myristic acid in sheep skin (8.94%) was about 3 times that of donkey skin (2.98%) and cow skin (3.03%). The relative content of palmitoleic acid in donkey skin and cow skin (9.98% and 13.46%) was about 3.6 times and 4.8 times, respectively, that of sheep skin (2.78%). Similarly, high differences were detected in the absolute contents of myritic acid and palmitoleic acid in donkey skin (2.98%, 9.98%), sheep hide (8.94%, 2.78%) and cow hide (3.03%, 13.46%). In summary, the establishment of the determination of the myristic acid and palmitoleic acid contents in donkey skin would provide a new idea for the rapid identification of authentic donkey skin.
