Whole-genome Sequencing of A Klebsiella pneumoniae ST37 Strain and Comparative Analysis of Virulence Factors
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Abstract:
In this study, a multi-drug resistant ST37 (Sequence type, ST) Klebsiella pneumoniae KP200 strain was isolated from the liver of sick swine. ST37 type is a common type among clinical isolates resistant to broad-spectrum β-lactam. Based on the whole-genome sequencing data, virulence factors carried by KP200 and all K. pneumoniae ST37 strains (160 strains) from NCBI were investigated, and the relationship between the virulence and genetic evolution of ST37 K. pneumoniae was analyzed by single-copy core gene evolution. The results showed that KP200 carried 7 types of virulence factor-related genes including capsule (cpsACP, galF, gnd, ugd, manB and manC), type 1 fimbriae (fimBEACDFGHK), type 3 fimbriae (mrkABCD), enterobactin (entABCDEF and fepABCDG), salmochelin (iroE and iroN), aerobactin (iutA) and bacterial Type VI secretion system T6SS (tssJFGKIBCDMHL). K. pneumoniae ST37 carrying more than 5 types of virulence factors accounted for 100%. The results of single-copy core gene evolution analysis showed that KP200 and the other 5 K. pneumoniae strains of human origin had a close relationship and similar virulence factors. This study showed that ST37 K. pneumoniae KP200 strains of swine origin carried a wide variety of virulence factors and had potential pathogenicity. There was an evolutionary relationship between to the virulence factors carried by ST37 K. pneumoniae and the evolution of single-copy core genes. The results of this study can provide basic data for the correlation between the virulence factors of ST37 K. pneumoniae and genetic evolution.
