Optimization of Microwave Extraction of Ficus carica Polysaccharide
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Abstract:
The microwave-assisted extraction method of Ficus carica fruit polysaccharides was studied. The Ficus carica polysaccharides were extracted by several steps, including microwave-assisted extraction, ethanol precipitation, Sevage deproteinization. Then polysaccharides content was determined by phenol-sulfuric acid method. The single factor experiments were used to investigate the influence of four factors, including microwave power, water-to-material ratio, temperature and microwave time on the yield of polysaccharides. On this basis, the orthogonal design of four factors and three levels of experiments were to study the influence of each factor on the extraction rate of Ficus carica polysaccharides, and the extraction conditions of Ficus carica polysaccharides were optimized by microwave-assisted method. The optimal extraction conditions were microwave power 600 W, water-to-material ratio 40 mL/g, temperature 70 ℃, and microwave time 40 min. The yield of polysaccharide was 12.17%, and it was 12.05% after repeated verification for 3 times. The results showed that microwave power was the main factor affecting the yield of polysaccharide. This study can provide a reference for further processing and utilization of Ficus carica.
