Interference Effect of Hemerocallis citrina Baroni Methanol Extract on Glycolipid Damage
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Abstract:
Caenorhabditis elegans (C. elegans) was used as a model organism to study the interference effects of methanol extract from Hemerocallis citrina Baroni on glycolipids damage. UPLC-QQQ-MS/MS was used to analyze the ingredients in methanol extract of Hemerocallis citrina Baroni, and based on the wild-type N2 C. elegans, sugar damage model was induced by 100 µmol/mL sucrose, lipid injury model was induce by100 µg/mL stearic acid. To observe the interference effects of methanol extract from Hemerocallis citrina Baroni, the three models were added with 0, 400, 800, 1200 µg/mL methanol extract of Hemerocallis citrina Baroni, egg production, body length and lifespan of C. elegans were detected. The results showed that the main component of methanol extract in Hemerocallis citrina Baroni was rutin with a content of 5721.11 ng/mg. For common N2 C. elegans, the total progeny and body length of the nematodes fed with 800 µg/mL Hemerocallis citrina Baroni methanol extract increased by 24.76% and 7.73%, respectively, compared with the blank group; for sugar-damaged C. elegans, fed with 800 µg/mL Hemerocallis citrina Baroni methanol extract, the lifespan and total progeny number of nematodes were increased by 24.47% and 3.40% respectively than those of the blank group; for lipid-damaged C. elegans, the lifespan, body length and total progeny number of nematodes fed with 1200 µg/mL Hemerocallis citrina Baroni methanol extract were increased by 30.04%, 10.36% and 97.32%than those of the blank group, respectively. Therefore, the results showed that the mid-to-high concentration of Hemerocallis citrina Baroni methanol extract had a certain interference effect on C. elegans glycolipids damage.
