Rapid Screening of 73 Pesticide Residues in Gleditsia Sinensis Seeds by Matrix Solid-phase Dispersion Extraction Combined with Gas Chromatography-mass Spectrometry
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Abstract:
A rapid screening method for 73 pesticide residues of different polarities in Gleditsia sinensis was established by matrix solid-phase dispersion extraction (dSPE) combined with GC-MS/MS analysis. The Gleditsia sinensis seed samples were extracted with acidified acetonitrile by shaking and ultrasonic-assisted extraction, and then added to the extraction bag for high-speed centrifugation. The supernatant was added to the purification tube and then subjected to dispersive solid-phase extraction purification. Multi-reaction monitoring scanning mode (MRM) was used for monitoring, and the matrix matching solution internal standard method was used for quantification. Each target had a good linear correlation in different ranges, with the correlation coefficients (R2) not lower than 0.990, and limits of quantification (LOQs) in the range of 2.5 ~ 20. 0 μg/kg (S/N=10). In the spiked Gleditsia sinensis seed matrix, the average recovery rates were 68.6%~110.7% with the relative standard deviations (RSDs, n=6) between 2.1% and 9.7%. Compared with the traditional pretreatment method, QuEChERS, the dispersive solid-phase extraction technology has certain advantages in detection cost and processing speed, and can analyze 73 pesticides simultaneously by one injection with high accuracy and sensitivity. This method can provide technical support for future research on the screening and confirmation of pesticide residues in food, risk assessment, and quantitative arbitration in the field of food risk monitoring.
