Cloning and Bioinformatics Analysis of DDE Transposase Gene from Streptomyces
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
In this paper, a DDE transposase-producing strain was selected from the strains preserved in the laboratory and indentified as Streptomyces labedae sp. X1 by morphology, physi-biochemistry and 16S rDNA. One 401 bp DDE transposase gene was amplified from the Streptomyces labedae sp. X1 genomic DNA, Blast and ISfinder results showed that it had 88% similarity with ISAzo13 family transposase genes. Bioinformatics analysis showed that the gene encoded 133 amino acids with stable hydrophilic structure, and the conserved amino acid triplet of Asp43, Asp49, Glu91 were found in the amplified DDE transposase; Based on the results of its α-spiral and irregular crimp, absence of signal peptides and transmembrane domains, it was a non-secreting protein with 14 phosphorylation and only one glycosylation site; and α-helix was the main composition in its advanced structure; SDS-polyacrylamide gelelectrophoresis showed that the molecular weight of the proposed protein was 27 ku. The results provide important information for the study of the expression mechanism of Streptomyces DDE transposase gene, and lay a foundation for the identification of DDE transposase activity, its structure and function in the future.
