Saccharomycete Fermenting Xylose to Ethanol and Its ARTP Mutation Breeding
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Abstract:
In order to improve the ability of saccharomycete to produce ethanol by xylose fermentation, the wild strain of Wickerhamomyces anomalus A42 was mutated by normal temperature and atmospheric pressure plasma technology (ARTP), and a high ethanol producing mutant strain with excellent performance was selected. The results showed that the best mutation condition was 120 s, under this condition, the lethal rate of A42 was 97.53%. The results showed that the ethanol yield of A42-338 was 42.59% higher than that of the original strain, and the ethanol content was 20.78 g/L after 60 h of fermentation, and the change rate of ethanol production of each generation was not larger than 2.50% after 8 passages. The results showed that the content of ethanol and reducing sugar of A42-338 were 19.88 g/L, 9.06 g/L, 1.79 g/L, 7.27 g/L, 84.61%, 81.05% and 0.40 g ethanol/g sugar respectively. Therefore, ARTP mutation is an efficient and feasible method for yeast breeding.
