Regulating Effects of Allyl Methyl Trisulfide on Inflammatory Factor and Intestinal Flora in Ulcerative Colitis Mice
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
The regulating effects of allyl methyl trisulfide (AMTS) on inflammatory factor and intestinal flora in ulcerative colitis mice were investigated in this study. Body weight, colon length, disease activity index (DAI) and histological changes were observed. Tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) contents in serum were detected by ELISA method. And the intestinal flora diversity in mice feces was analyzed by 16s rDNA high throughput sequencing. The results showed that the weight of the model group decreased by 25.59%, and the low-dose, medium-dose and high-dose AMTS groups were decreased by 4.06%, 9.88% and 5.45% respectively. The DAI in model group was 3.51, the three AMTS groups were 2.60, 2.88 and 2.79, respectively. Compared with the model group, three dosages of AMTS groups ameliorated inflammatory infiltration in colonic tissue, and significantly decreased the levels of pro-inflammatory cytokines, like TNF-α, IL-1β, IL-6 (p<0.01, p<0.05). The abundance and diversity of intestinal flora were increased after administration with AMTS. Compared to the model group, the microbial abundance of Bacteroidetes, Proteobacteria were decreased, and the abundance of Firmicutes, Lactobacillus were increased in the AMTS groups. In conclusion, the results indicate that AMTS alleviates the symptoms of ulcerative colitis by reducing the level of inflammatory factors and increasing the abundance and diversity of intestinal flora in mice.
