Palmitic Acid Disrupts the Growth, Development and Glucose and Lipid Metabolism of Drosophila melanogaster
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Abstract:
In order to explore whether or not palmitic acid (PA) causes metabolic syndrome, Drosophila melanogaster was used as the model organism in this study, corn flour yeast standard medium was used as the control group, and the standard culture medium added with 2.50% PA as the experimental group. The larval body weight, pupation rate and eclosion rate, pupal weight, pupal area, pupal volume, adult body weight, adult crawling ability, adult wing area, and the contents of trehalose, triglycerides, Dilp2, Dilp3 and Dilp5 of the third instar larvae of D. melanogaster were determined. The results showed that compared with CTRL group, PA decreased the pupation rate and eclosion rate of D. melanogaster by 3.75% and 10.03%, respectively, with the pupal area and pupal volume of the PA group also decreased. The body weights of female drosophila and male drosophila in the PA group decreased by 0.13 mg and 0.10 mg, respectively, compared with CTRL group. The locomotor activities of female drosophila and male drosophila in the PA group decreased by 41.35% and 22.78%, respectively. For the PA group, the wing area of female drosophila in decreased significantly, but there was no significant change in male drosophila. The contents of trehalose and triglycerides in the PA group increased significantly, while the expressions of Dilp2, Dilp3 and Dilp5 decreased significantly. The above results indicate that PA inhibited the growth and development of D. melanogaster and disrupted the balance of glucose and lipid metabolism in larvae. This study can provide a scientific basis for exploring the mechanism of the metabolic syndrome caused by a high-fat diet.
