Screening for Cholesterol-lowering Lactobacillus reuteri Strains and Analyzing Their Probiotic Effectiveness
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Abstract:
To obtain probiotic strains with significant cholesterol-lowering performance, strains were isolated from quark produced at the Litongda Pasture in Manzhouli, Inner Mongolia. The cholesterol-lowering ability of each strain was determined by the o-phthalaldehyde method, and the strains with strong cholesterol-lowering abilities were selected. The strains were identified based on the morphology, physiology, biochemistry, and 16S rDNA sequences, and their tolerance for acids, bile salts, and gastrointestinal juice was determined. Finally, five cholesterol-lowering strains were obtained, and one of them showed the best ability to lower the cholesterol level, with a degradation rate of 50.60%. This strain was identified as Lactobacillus reuteri, and named as HCS02-001 (CGMCC No. 19746). The results of the various tolerance tests were as follows. When the strain was cultured at pH 3.0 and pH 2.0 for 17 h, the final survival rates were 86.80% and 85.52%, respectively. Similarly, the survival rates were 97.42% and 95.17% after the strain was cultured for 17 h in 0.3% and 0.5% bile salts, respectively. In addition, the survival rates were as high as 96.74% and 98.41% after three hours of treatment in artificial gastric juice and four hours of treatment in artificial intestinal juice, respectively. In summary, a strain of Lactobacillus reuteri with good cholesterol-lowering performance and showing high tolerance for acids, bile salts, and gastrointestinal juices, had been identified. These findings are of great relevance to the research and development of cholesterol-lowering products.
