Abstract:Whole-genome sequencing of Lactiplantibacillus plantarum DMDL 9010 (LP9010) was first performed, and its corresponding NCBI accession number is CP063986-CP063988. The nitrite degradation performance of LP9010 was preliminarily investigated based on the changes in the physicochemical indicators under different cultivation conditions and medium compositions, which was followed by the whole-genome sequencing analysis. The results revealed that LP9010 can effectively degrade nitrite. The nitrite degradation rate of LP9010 increased with a decrease in the pH. The temperature of 37 ℃ was conducive for nitrite degradation by LP9010. The optimal nitrite degradation performance of LP9010 can be achieved when there is sufficient nutrition, but oxygen and growth factors do not exert significant effects on nitrite degradation (p>0.05). Furthermore, acid tolerance-related genes (nhaC, gadA, argG, and glnR), cold- and heat-tolerance-related genes (cfa, cspA, hrcA, htpX, cplC, clpE, clpP, clpX, and clpL), oxygen tolerance-related genes (catB, trxB, trxA, gor, msrC, msrA, and msrB), and sugar transport-related genes (pstI, Crr, NagE, ScrA, MtlA, ManX, ManY, ManZ, GatA, GatB, GatC, FruA, and FruB) were found in the whole genome sequencing of LP9010. Besides, the nitrite reductase genes (lmrB and pgl) were discovered. Conclusively, LP9010 can degrade nitrite efficiently and it possesses the genes related to nitrite degradation. This article provides a new direction to better understand and elucidate the nitrite degradation mechanism of Lactiplantibacillus plantarum.
