QuEChERS-Gas Chromatography Tandem Mass Spectrometry for the Determination of Vinclozolin and Its Metabolite Residues in Fruits and Vegetables
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Abstract:
An improved QuEChERS method combined with gas chromatography/mass spectrometry (GC/MS) method was established for the rapid determination of vinclozolin and its metabolite, 3,5-dichloroaniline residue, in fruits and vegetables. This research improved the type and amount of adsorbent in QuEChERS method. Vinclozolin and its metabolite residues in tomatoes, cucumbers, lettuce, celery, citrus and grapes were extracted with ethyl acetate. The extract was purified using 80 mg of PSA and 50 mg of GCB to remove impurities via adsorption, before quantification by GC/MS with external standards. The results showed that vinclozolin and its metabolite 3,5-dichloroaniline showed a linear relationship in the mass concentration range of 0.02~0.25 mg/mL, with the correlation coefficients as 0.997 and 0.998 respectively. The average recovery range of vinclozolin and its metabolite in tomatoes, cucumbers, lettuce, celery, citrus and grapes was between 81.67% and 113.33% at the spiked level of 0.05~3.0 mg/kg, with the relative standard deviations (RSDs) less than 9.47%. The limits of detection (LOD, S/N=3) were between 4.2 and 15.5 μg/kg for vinclozolin, and between 6.3 and 17.6 μg/kg for 3,5-dichloroaniline. This method is rapid, simple and sensitive, and can meet the detection requirements of residual vinclozolin and its metabolites in fruits and vegetables.
