Rapid Analysis of 13 Kinds of Plant Hormones in Rice by Ultra-performance Liquid Chromatography-tandem Mass Spectrometry
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Abstract:
A rapid and efficient method based on ultra-performance liquid chromatography-tandem mass spectrometry was established for the analysis of 13 plant hormones in rice. The conditions for chromatographic separation and mass spectrometry detection were optimized. The target compounds were separated by ACQUITY UPLC BEH C18 (2.1 mm × 100 mm, id 1.7 μm) with gradient elution using acetonitrile (0.04% V/V formic acid) and water (0.04% V/V formic acid) solutions as the mobile phase; baseline separation was achieved within 11 min. The 13 plant hormones were determined in the electrospray positive ionization (ESI+) and multiple reactions monitoring (MRM) modes. Analysis of the results indicated that the 13 plant hormones had good linearity in the concentration range from 1 to 500 ng/mL, and the correlation coefficients were all greater than 0.9980; the recoveries ranged between 81.9% and 118.8%, and the relative standard deviations (RSD, n=3) were between 2.45% and 8.12%. The method is simple, sensitive, accurate, and reliable; therefore, it is suitable for determining the contents of 13 kinds of plant hormones in rice. Furthermore, it can provide technical support for detailed examination of the growth and development process of rice and the identification of plant hormone residue limits.
