Determination and Comparation of the Volatile Components in Three Kinds of Characteristic Honeys of Yunnan Province
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Abstract:
In order to explore the composition and difference of the volatile components in three characteristic honeys from Yunnan province, headspace solid phase microextraction coupled to gas chromatography-mass spectrometry was used to determine the volatile components of the three honeys (Vicia villosa Roth honey, Macadamia integrifolia honey, and Hevea brasiliensis honey), combined with principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). The results showed that a total of one hundred and five volatile compounds were detected in the three honeys, with 61 in Vicia villosa Roth honey, 55 in Macadamia integrifolia honey, and 62 in Hevea brasiliensis honey. Aldehydes, acids and alcohols were the main volatile compounds in Vicia villosa Roth honey (n-nonanal and acetic acid had higher contents). Alcohols terpenes and aldehydes were the main volatile compounds in Macadamia integrifolia honey (cis-linalool oxide and furanoid linalool oxide had higher contents, with trans- linalool 3,7-oxide as the characteristic volatile component). Aldehydes, alcohols and acids were the main volatile compounds in Hevea brasiliensis honey (nonaldehyde and trans-linalool oxide were of higher contents). The results of PCA and PLS-DA analysis showed that there were significant differences in volatile components among Vicia villosa Roth honey, Macadamia integrifolia honey, and Hevea brasiliensis honey, and the three kinds of honey were well classified with a 100% accuracy. The research showed that Vicia villosa Roth honey, Macadamia integrifolia honey and Hevea brasiliensis honey have their own unique volatile components, and the PCA and PLS-DA models established based on these volatile compounds can realize the effective classification and identification of the three honeys in Yunan.
