Optimization of Extraction Process of Russula virescens Polysaccharide
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Abstract:
The ultrasonic-assisted extraction method of Russula virescens polysaccharide was studied. Several steps were used to extract Russula virescens polysaccharides including ultrasonic-assisted extraction, ethanol precipitation, Sevage deproteinization, then polysaccharide content was determined by phenol-sulfuric acid method. In this paper, single factor experiments were used to investigate the influence of four factors, including ultrasonic power, temperature, ultrasonic time and water-to-material ratio, on the yield of polysaccharides. On this basis, Box-Benhnken response surface method was used to design a four-factor three-level experiment, and a regression equation was established to study the degree of influence of each factor on the yield of Russula virescens polysaccharides. The extraction process was further optimized and the optimal extraction conditions for Russula virescens polysaccharides were obtained. The optimal extraction conditions were ultrasonic power 500 W, temperature 76 ℃, ultrasonic time 40 min, and water-to-material ratio 31 mL/g. The yield rate of polysaccharide was 6.50%. Therefore, the ultrasonic-assisted method is helpful to improve the extraction rate of Russula virescens polysaccharide. The regression model established by response surface method and the correlation between the actual value and the predicted value of related parameters are better, which can be used to analyze and predict the ultrasonic extraction of Russula virescens polysaccharides.
