Optimization of the Purification Process for the Polysaccharides from Tetradium ruticarpum Fruit Using Macroporous Adsorption Resins
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Abstract:
In order to obtain the best purification process using macroporous resin for the polysaccharides from Tetradium ruticarpum fruit (TrPs), water extraction and alcohol precipitation were applied to extract the polysaccharides. The purification performance of seven different types of macroporous adsorption resins was examined by used static and dynamic single factor experiments, and the purification process was optimized by the response surface method. Finally, the polysaccharide structure before and after adsorption was characterized. The results show that the S-8 resin had the highest comprehensive purification efficiency for the crude TrPs, and the optimal purification conditions were: sample concentration, 4.41 mg/mL; initial pH 5; adsorption environment temperature, 24.30 ℃; flow rate 2.0 BV/h. Under these conditions, the decolorization rate was 49.21%, deproteinization rate was 68.97% and polysaccharide retention rate was 55.05%. The structural characterization results revealed the molecular weight (Mw) distribution of the polysaccharide before and after adsorption (Mw of peak 1 higher than 2.00×105 u; Mw of peak 2, approximately 2.35×104 u), the infrared absorption characteristics and the sugar chain anomeric hydrogen absorption signal of TrPs (δ 5.04×10-6 and δ 4.42×10-6) did not change significantly before and after adsorption. In summary, S-8 macroporous adsorption resin can be used for the high-efficiency purification of TrPs, under mild and environmentally friendly conditions, without causing polysaccharide chain scission and polysaccharide structural changes.
