Inhibitory Effects of Phenolic Acid Monomers in Sugarcane Molasses on PhIP in a Phenylalanine-creatinine Model Reaction System
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Abstract:
The inhibitory effect of ferulic acid and vanillic acid in sugarcane molasses on PhIP was studied in a model reaction system. The colloid, ash and heavy metals in the molasses were removed through the molasses purification treatment method to yield a crude molasses extract. A gallic acid standard curve was established to determine the polyphenol content in molasses extract. The type of phenolic acids in the molasses extract was determined by UPLC-MS analysis. The phenolic acid standard was added to the model reaction system (phenylalanine and creatinine), and changes in PhIP in the model reaction system was analyzed by the UPLC-MS/MS system. The results showed that the polyphenol content in the molasses extract was as high as 3.58 mg/g. Compared with the phenolic acid standard, the molasses extract was found to contain ferulic acid and vanillic acid. In the model reaction system, withthe increase of the concentration of vanillic acid or ferulic acid, both the phenylalanine content and the amount of PhIP produced in the model reaction first decreased gradually and then leveled off. When the concentration of ferulic acid reached 2.33×10-7 g/mL, the inhibitory effect on PhIP was the best, and the overall inhibitory rate was 76.67%. When the concentration of vanillic acid reached 2.02×10-7 g/mL, the inhibitory effect on PhIP was the greatest, and the overall inhibitory rate reached 77.43%. In summary, the ferulic acid and vanillic acid monomers contained in sugarcane molasses had strong inhibitory effects on PhIP in the model reaction system, which provides a basis for subsequent research on the inhibitors of PhIP.
