Abstract:Differences between extracellular metabolites of recombinant strain N6076 mutagenized by low-energy ion beams and original strain Kh08 at different fermentation stages were studied by metabolomic methods. The related metabolic pathways were discussed based on network pharmacology. The results show significant differences between the extracellular metabolites of the two strains at different stages. Fifteen metabolites with VIP>1 were identified including indole-3-propionate (IPA), a common metabolite showing the most significant difference. The pathways of metabolites showing differences were examined based on network pharmacology. It has been found that four metabolic pathways of the recombinant strain show significant differences, namely lipid metabolism, phosphatidylinositol (GPI)-anchoring, pantothenic acid and CoA biosynthesis, and the glycerophospholipid metabolism. This study provides scientific evidence and understanding of the metabolic differences of recombinant yeast and a possible direction for future strain improvement.
