Functional Analysis of Squalene Monooxygenase Gene for the Carotenoid Biosynthesis in Cordyceps militaris
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Abstract:
Based on transcriptome data, it was speculated that erg1 gene may be involved in carotenoid synthesis in Cordyceps militaris. Under the stress factors of KMnO4 and NaCl, the mRNA relative expression level of erg1 gene was further detected and analyzed to explore its regulatory role in carotenoid synthesis pathway. In order to verify erg1 gene function, an over-expressing vector pCambia0390- blpR-Pcmgpd-erg1of the erg1 gene was constructed. Subsequently, the expression cassette was introduced into the C. militaris by Agrobacterium tumefaciens. Finally, the expression level of erg1 gene and the amount of carotenoid biosynthesis were determined. The results indicated that the carotenoid production of recombinant strain Cmerg1 under KMnO4 (oxidative) and NaCl (osmotic) stress reached 6757.17 μg/g and 6363.93 μg/g respectively, which were 1.22 and 1.20 times of the control strain CM10, respectively. In conclusion, the overexpression of erg1gene caused the increase of carotenoid production of Cmerg1 strain. In other words, the squalene monoxygenase gene is involved in the carotenoid synthesis of C. militaris. The results laid the experimental foundation for the research and utilization of carotenoid biosynthesis in C. militaris.
