Sinapic Acid Ameliorated Lipid Metabolism and Oxidative Stress in High Fat Diet Fed Syrian Hamsters
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Abstract:
To investigate the effect of sinapic acid on lipid metabolism and oxidative stress in a high-fat diet (HFD) fed Syrian hamsters, the serum and hepatic levels of total cholesterol (TC), triglycerides (TG), high density lipoprotein (HDL-c), low densitylipoprotein (LDL-c), nonesterified free fatty acids (NEFA) and malondialdehyde (MDA) and total antioxidant capacity (T-AOC) were determined by colorimetric method. The hepatic levels of lipid metabolism related proteins, which including peroxisome proliferator-activated receptor gamma (PPAR-γ) carnitine palmitoyltransferase-1 (CPT-1), acetyl-CoA carboxylase1 (ACC1), fatty acid synthase (FAS), 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), sterol regulatory element binding protein-2 (SREBP2) and cytochrome P450 family 7 subfamily A member 1 (CYP7A1) were determined using a Western Blotassay. Administrated with sinapic acid can significantly reduce the weight of HFD hamsters (23.86%), epididymal fat (20.38%) and perirenal fat (27.91%) (p<0.05) compared with HFD fed hamsters. Sinapic acid treatment also attenuated the serum lipid levels (TG: 183.51 μmol/L, TC: 1481.67 μmol/L, HDL-c: 507.81 μmol/L, LDL-c: 272.50 μmol/L and NEFA: 1.16 ng/L). The MDA levels were reduced to 7.85 nmol/L and 5.47 nmol/L, and T-AOC levels were increased to 12.63 U/mL and 113.72 U/mL in serum and liver of the model hamsters, respectively, by the intervention of sinapic acid. In addition, sinapic acid can promote the protein expression of PPAR-γ (2.00 times), CPT-1 (2.61 times), CYP7A1 (1.65 times) and inhibit the protein expression of FAS (34.38%), ACC1 (52.73%), SREBP2 (53.49%) and HMGCR (32.68%) compared with HFD hamsters. Our results suggested that sinapic acid can significantly improve lipid metabolism disorders induced by HFD in hamsters, which is related to the regulation of lipid metabolism-related proteins and the reduction of oxidative stress levels.
