Analysis of Virulence and Drug Sensitivity of Vibrio parahaemolyticus in Aquatic Products
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Abstract:
In order to investigate the virulence gene carrying status and drug sensitivity of Vibrio parahaemolyticus in aquatic products, 60 strains of Vibrio parahaemolyticus were isolated and identified from aquatic products by the isolated cultivation method from GB 4789.7-2013, VITEK 2 compact automatic bacteria identification system and real-time PCR. The distribution of 4 virulence genes of Vibrio parahaemolyticus isolates was detected by conventional PCR. Meanwhile, drug susceptibility of the isolates was tested by using VITEK 2 compact automatic bacteria identification system. Results showed that in 60 strains of Vibrio parahaemolyticus isolated from commercial aquatic samples, the gene carrying rates of tlh, toxR/S, tdh, trh were 100.00%, 100.00%, 95.00%, and 65.00%, respectively, and quad-virulence genes carrying rate was 61.67%. Results of drug susceptibility test using 19 types of antibacterial agents indicated that all of the 60 isolates were highly resistant to ampicillin, and the resistance rate was 96.67%. The phenomenon of multiple drug resistance was not prominent, for just only 4 isolates were resistant to 2 drugs. The results indicated that the virulence gene carrying rate of Vibrio parahaemolyticus from the commercial aquatic samples was high, and most of the isolates carried multiple virulence genes. The drug resistance of Vibrio parahaemolyticus from the commercial aquatic samples was not outstanding, but the resistance rate to ampicillin was high, so we should be alert to the potential threat of Vibrio parahaemolyticus in aquatic food.
