Kinetic Characterization and Immobilization of Tannase Produced by Aspergillus niger N5-5
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Abstract:
Tannase produced by Aspergillus niger N5-5 has been independently isolated in our laboratory, and proven to efficiently hydrolyze propyl gallate. To explore industrial applications for tannase, we cultivated large quantities of tannase and investigated the kinetics of its hydrolysis of tannin, and the effects of immobilization on its enzymatic activity. Fermentation began with growth in liquid media, followed by fermentation on solid media. After enzyme extraction, ceramic membrane filtration was used to purify and concentrate the enzyme. Two different drying methods, (lyophilization and spray drying) were adopted. Activated resins were used to immobilize tannase. The purified tannase solution has an enzymatic activity of 258.53 U/mL. It can hydrolyze 10%~50% tannic acid solutions, and its performance is better when substrate concentrations are less than 30%. Lyophilization has little effect on enzyme activity, while spray drying decreases enzyme activity to 174.02 U/mL. In addition, tannase immobilized on resin can be reused at least 4 times under experimental conditions. This research has established a theoretical basis for improving production efficiency of tannase from Aspergillus niger N5-5, reducing the cost of enzymatic hydrolysis, and realizing commercial enzyme production.
