Preventive Effect of an Aqueous Extract from Leaves of Panax notoginseng on 5-Fluorouracil-Induced Intestinal Mucositis in Mice
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Abstract:
The objective of this study was to explore the preventive effect of an aqueous extract of Panax notoginseng leaves on 5-fluorouracil (5-FU)-induced intestinal mucositis. Kunming male mice were selected. Each group was administered intragastrically before 5-FU was injected intraperitoneally to establish an intestinal mucositis model. The changes in the body weights of mice were recorded. The morphological changes of the jejunum were examined by HE staining. The expression of proliferating cell nuclear antigen (PCNA) in the jejunum was examined by immunohistochemistry. The mRNA expressions of MUC2, ZO-1, Occludin, IL-1β, IL-6 and TNF-α were determined by real-time fluorescence quantitative polymerase chain reaction. Compared with the model group, the body weights of the mice in the high-dose group increased by 49.24%, and their jejunum villus length increased by 22.92%, crypt depth decreased by 16.27%, villus length-to-crypt depth ratio increased by 42.32%, and expression of PCNA in the jejunum increased by 21.54%. The mRNA expressions of MUC-2, ZO-1 and Occludin in jejunum of high-dose group increased by 157.66%, 167.73% and 212.14% respectively, the mRNA expression of IL-1β, IL-6 and TNF-α in the jejunum of the high-dose group decreased by 34.24%, 35.18% and 33.52% respectively. The above-described results showed that the aqueous extract of Panax notoginseng leaves could prevent 5-FU-induced intestinal mucositis in a concentration-dependent manner.
