In Vitro Digestion Properties of Polygonatum sibiricum Polysaccharide and Its Regulatory Action on the Gut Microbiota in T2DM Mice
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Abstract:
In this study, the in vitro digestion characteristics, in vitro hypoglycemic activity and regulatory action of Polygonatum sibiricum polysaccharides towards gut microbiota in type 2 diabetes (T2DM) mice were investigated. With the blank as a control, Polygonatum sibiricum polysaccharide was subjected to in vitro digestion, and the changes in the content of reducing sugars and molecular weight of Polygonatum sibiricum polysaccharides during the digestion were evaluated by the DNS method and gel chromatography. The in vitro fermentation of Polygonatum sibiricum polysaccharide was conducted, and the resulting products were subjected to 16S rDNA analysis to examine the effect of Polygonatum sibiricum polysaccharide on gut microbiota of T2DM mice. The obtained results showed that During the simulated stomach digestion, the molecular weight of Polygonatum sibiricum polysaccharides decreased from 24.19 ku to 20.39 ku, and during the simulated intestinal digestion, the molecular weight decreased from 28.85 ku to 24.35 ku. These results showed that most of Polygonatum sibiricum polysaccharides could reach the end of intestine after digestion and could be utilized by gut microbiota, with a small amount being directly digested and absorbed. Through the analysis of α diversity and β diversity based on the 16S rDNA sequencing analysis of the bacteria in the fermentation liquid, Polygonatum sibiricum polysaccharides were found to increase significantly the abundance and diversity of the gut microbiota and change the composition of the microbiota. At the phylum level, Polygonatum sibiricum polysaccharides significantly changed the relative abundance of the dominant phylum Firmicutes, Proteobacteria and Bacteroides. At the genus level, Polygonatum sibiricum polysaccharides changed significantly the relative abundance of dominant genus such as Lactobacillus, Veillonella, Escherichia and Klebsiella. The results showed that Polygonatum sibiricum polysaccharides could improve the condition of diabetic mice through regulating their gut microbiota.
