Protective Effect of the Enzymatic Peptide from Deer Antler Blood Against Lipopolysaccharide-induced Cardiomyocyte Damage in H9c2 Rats
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Abstract:
In order to investigate the protective effect of the enzymatic peptide from deer antler blood aginst lipopolysaccharide (LPS)-induced H9c2 rat cardiomyocyte damage, this study used LPS to stimulate H9c2 cells to establish an injury model. Captopril was selected as the positive control drug. MTT method was used to determine the effects of different concentrations (25, 50, 100, 200, 400 μg/mL) of the enzymatic peptide from deer antler blood on H9c2 cell proliferation-inhibitory activity; Enzyme-linked immunoassay was used to determine the contents of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β) in the cell supernatant. The amino acid composition of the enzymatic peptide from the antler blood was analyzed. The results showed that compared with the model group, different concentrations (25, 50, 100, 200, 400 μg/mL) of deer antler hemolyzed peptides could significantly inhibit the proliferation of damaged H9c2 and the release of TNF-α, IL-6 and IL-1β (p<0.05). The contents of the released TNF-α, IL-6 and IL-1β in the model group were 531.05, 185.41, and 70.03 (pg/mL), respectively. At a mass concentration of 200 μg/mL, the enzymatic peptide from the deer antler blood exhibited the greatest inhibitory effect on the release of the three above-mentioned inflammatory factors (p<0.001), with the released amounts as 357.93, 148.69, and 62.72 (pg/mL), respectively. Lys was rich in the enzymatic peptide from the antler blood that exhibited protection on the injured H9c2 cells induced by LPS, accounting for 17.81% of the total amino acids. These results indicate that the enzymolyzed peptide from deer antler blood can inhibit the proliferation of injured H9c2 cells, while reducing the release of inflammatory factors TNF-α, IL-6 and IL-1β, and exert its effect on LPS-induced H9c2 cells through inhibiting the secretion of inflammatory factors.
