Rapid Detection of Terbutaline Sulfate in “Lean Meat Powder” Based on SERS
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Abstract:
A method for rapid detection of terbutaline sulfate by surface enhanced Raman spectroscopy (SERS) was developed. Firstly, the surface-enhanced active substrate was prepared, and the enhancer was characterized by electron microscopy and UV-Vis absorption spectroscopy. The experimental results showed that the prepared silver sol exhibited the greatest enhancing effect on the Raman signal information of terbutaline sulfate, thus, the silver sol was selected as the surface enhancer. A portable Raman spectrometer was used to detect terbutaline sulfate in water and urine. The Raman signals at 800 cm-1, 1050 cm-1, 1330 cm-1 and 1450 cm-1 were identified as the Raman characteristic peaks of terbutaline sulfate. A good linear relationship between the signal intensity of surface enhanced Raman spectrum and the concentration of terbutaline sulfate in the range of 1~10 mg/L was obtained through further experiments. The correlation coefficient R2 was 0.9718, with the detection limit as 0.5 mg/L and the detection time as 60 s. The recoveries of the blank samples were 84.9%, 91.6% and 89.1% in the three-levels standard spike-and-recovery experiments. These results were in good agreement with those of comparative experiments by high performance liquid chromatography-based traditional large-scale analysis instrument methods. The results of this research showed that the method is advantageous as it is fast, accurate, non-destructive and easy to operate, which laid a good foundation for the supervision of “lean meat powder”.
