Comparison of Extraction Effect of Several DNA Extraction Methods in Tuna Products
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Abstract:
There are many types of tuna, shoddy and fake tuna products are found in the market from time to time. DNA-based detection technologies have been widely used in the detection of adulteration of tuna products. DNA extraction is crucial to the application of DNA detection technology. In this study, 28 deep-processed tuna products were used as the experimental samples. Three different DNA extraction methods (SDS method and two commercially available kits) were compared in terms of DNA quality, yield, PCR amplification and method operability. The results showed that all the three methods can be used for DNA extraction from most tuna products. Compared with the other two commercially available kits, the SDS method led to a lower DNA yield with a higher purity and a A260-to-A280 ratio (1.89), less salt residue, lower inhibition in real-time fluorescent quantitative PCR and relatively low cost, which meets the demand for adulteration detection of tuna products.
