Antiallergic Activity and Mechanism of Ethanol Extract from Perilla Leaves
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Abstract:
Objective: to investigate the anti allergic activity and mechanism of ethanol extract from Perilla frutescens leaves in vivo. Methods: passive skin anaphylaxis test was carried out in mice. The serum levels of immunoglobulin E, leukotriene B4 and platelet activating factor in mice were determined to explore the anti allergic effect and mechanism of perilla leaf ethanol extract on type I hypersensitivity. To explore the antiallergic effect of ethanol extract from Perilla frutescens leaves on type IV anaphylaxis, DNCB induced delayed type hypersensitivity test was carried out in mice. Results: In the passive skin anaphylaxis test of mice, compared with the model group, the high-dose ethanol extract of perilla leaves significantly reduced the itching times of mice (p<0.01), from 24.3 times to 15.8 times. The first itching time was significantly prolonged from 121.59 s to 231.90 s. The levels of IgE, PAF and LTB4 in serum of high dose group decreased by 30.92%, 15.5% and 22.45%, respectively; compared with the DNCB-induced delay model group, the high-dose ethanol extract of perilla leaves significantly reduced the ear swelling degree of mice on the 3rd and 6th days (p<0.05) hypersensitivity in mice, on the 9th day, the ear swelling was significantly reduced (p<0.01). The levels of IL-4, IFN-γ and TNF-α in high-dose group decreased by 19.14%, 32.52% and 16.61% respectively. In conclusion, the extract of Perilla frutescens has obvious anti allergic effect on type I and type IV allergy. Its anti allergic mechanism to type I allergy is related to preventing the release of bioactive substances and interfering with the production of arachidonic acid metabolites.
