Advances on Detection Techniques for Foodborne Listeria monocytogenes
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Abstract:
Listeriosis caused by Listeria monocytogenesis currently considered to be one of the leading foodborne diseases worldwide, with a mortality rate of 20~30%. L. monocytogenes can be found in most foods and proliferate in a wide range of pH and osmolarity, at low temperatures, and under aerobic and anaerobic conditions. Thus, effective monitoring and control over L. monocytogenes along the food chain are necessary to prevent food contamination and outbreak of foodborne diseases. Foodborne L. monocytogenes is a low-cell-count pathogenic bacterium similar to other non-pathogenic Listeria regarding colony morphology and physicochemical characteristics, which makes its detection challenging. This study comprehensively reviewed the traditional culture methods and emerging alternative protocols, such as immunoassays, molecular bioassays, biosensors, bacteriophage-based methods, and compared their respective advantages and disadvantages, so as to provide a reference for rapid detection of L. monocytogenes in food.
