Abstract:In order to study the transfer and distribution of tetrodotoxin (TTX) in Takifugu obscurus and its activity on liver metabolizing enzymes, the oral gavage administration method was used in the experiment. The concentration changes of TTX in different tissue were measured by ultra performance liquid chromatography tandem mass spectrometry, and the activity of metabolic enzymes in the liver was also measured. The results were as follows, during the short-term metastasis distribution period after TTX instillation, the concentration of TTX in the gonads increased from 19.00 ng/g to 42.02 ng/g, and the accumulation rate was 121%. The TTX concentration was fluctuated in the liver. At 4 h, the highest TTX concentration was 11.43 ng/g, at 48 h, the lowest TTX concentration was 1.27 ng/mL. The TTX contents in the tissues of skin, muscle, blood, intestine, and gills were highest at 2 h, which were 7.43 ng/g, 1.15 ng/g, 11.44 ng/g, 9.77 ng/g, and 31.75 ng/g, respectively. The highest TTX concentrations in the spleen, kidney and gallbladder tissues were 5.05 ng/g, 6.54 ng/g, and 9.06 ng/g at 2 h, 4 h, and 6 h, respectively. In addition, the test results of liver metabolic enzyme activity showed that TTX significantly increased the activities of lactate dehydrogenase, aspartate aminotransferase and alanine aminotransferase (p<0.05). And it had a significant inhibitory effect on two phosphatase activities in the early stage (p<0.05), and then returned to normal levels. Theresults indicate thatthe transfer and distribution of TTX in Takifugu obscurus has tissue specificity, and can promote and inhibit the activities of different metabolic enzymes in the liver.
