Optimization of Culture Medium for High-yield Astaxanthin in Haematococcus pluvialis
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Abstract:
In this study, an optimal medium for cell proliferation of Haematococcus pluvialis HP1 during the green cell growth stage was selected from five media. Furthermore, the organic and inorganic carbon and their concentrations in the selected medium were also optimized respectively. Based on this, the single factor experiment and central composite design (CCD) response surface analysis were utilized to optimize the stress condition of astaxanthin accumulation with light intensity, initial pH value and inducement time as experimental factors. The results indicated that the biomass of HP1 could reach a higher level in medium A and OHM, and the optimal concentration of organic carbon source and inorganic carbon source was 0.5 g/L. The optimal stress conditions for HP1 astaxanthin accumulation were: light 145 μmol/m2·s, initial pH 7.0, and induction time 9 d. Under the conditions described above, the maximum yield of astaxanthin reached 5.74 mg/L in NaAC culture system and 4.25 mg/L in NaHCO3 culture system, respectively. This study can provide a scientific basis for further increasing the astaxanthin yield of Haematococcus pluvialis.
