Chitooligosaccharides Regulate Dyslipidemia and Oxidative Stress in NOD Mice
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Abstract:
To explore the possible mechanism underlying the ability of chitooligosaccharides (COS) to delay the progression of spontaneous diabetes in NOD mice, the effects of different doses of COS on dyslipidemia and oxidative stress were studied. After continuous intragastric administration of different doses of COS for 28 weeks, the indicators including blood glucose, blood lipids and oxidative stress of mice in each group were determined, and the histological examination was carried out. The results showed that COS at each dose could delay the time for NOD mice to enter the prediabetic and diabetic phases, and extend the time period of prediabetic phase to 10 W, 13 W and >16 W, respectively (9 w for the control group); COS at the three doses could decrease the level of T-CHO by 37.10%, 46.68% and 62.72%, respectively (p<0.01), level of TC by 35.01%, 45.06% and 60.62%, respectively (p<0.01), and level of LDL-C by 31.21% (p<0.05), 51.68% and 72.48%, respectively (p<0.01), although insignificant effect of COS on HDL-C was found. These results indicate that COS can improve dyslipidemia, increase the specific activities of SOD and GSH-Px in the liver tissues of mice (especially increased by 94.69% and 167.59%, respectively, in the COS-H group; p<0.01), and decreased the MDA concentration (by 48.41%; p<0.05), thereby reducing effectively oxidative stress-induced oxidative damage. Histological examination showed that COS increased the pancreatic coefficient, decreased gradually the liver coefficient, reduced fat vacuoles, providing a certain protective effect on the integrity of pancreatic tissue. According to these results, COS can delay the development of spontaneous diabetes through regulating dyslipidemia, resisting oxidative stress in the body caused by abnormal blood sugar, and protecting the tissues.
