Protective Effect of Total Flavonoids of Penthorum chinense Pursh on Acute Chemical Liver Injury Induced by Carbon Tetrachloride in Mice
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Abstract:
To study the protective effect of total flavonoids of Penthorum chinense Pursh on acute chemical liver injury induced by carbon tetrachloride in mice, in this study, 50 SPF Kunming mice were divided into five groups including normal group, model group, bifendate dropping pills group (150 mg/kg), total flavonoids of Penthorum chinense Pursh in high and low dose groups (800, 400 mg/kg). The mice were intragastrically administered with drugs or distilled water according to the body weight once a day for 21 days. One hour after the last administration, the other groups except the normal group were treated with the intraperitoneal injection of 1% CCl4 olive oil solution (10 mL/kg). Then all mice were killed after 16h, and the serum was collected for the determination of ALT, AST, ALP and LDH levels by automatic biochemical analyzer. Liver was triturated for detecting TNF-α, IL-6, SOD and MDA by ELISA method. The pathological changes of liver tissue were observed by HE staining. Compared with the model group, the activities of serum ALT, AST, ALP and LDH in high dose group were respectively reduced by 86.01%, 91.03%, 25.50% and 95.54%, while in low dose group were respectively decreased by 76.06%, 84.91% and 93.87%; in high dose group, the contents of TNF-α, IL-6 and MDA in liver tissue were respectively decreased by 24.89%, 20.91% and 39.45%, while SOD activity increased by 48.22%; in low dose group, the contents of IL-6 and MDA in liver tissue were respectively decreased by 18.60% and 25.62%, while SOD activity increased by 42.23%; and the pathological conditions of liver were significantly improved in high and low dose groups. The results suggest that total flavonoids of Penthorum chinense Pursh exhibits the protective effect on acute chemical liver injury induced by CCl4 in mice, and the mechanism of action may be associated with its inhibition of the oxidative stress reaction and the inflammatory reaction.
