The Combination of Oyster Peptides, Soybean Peptides, Collagen Peptides, and Perilla Seed Peptides Promotes the Production of Male Hormones in Testicular Stromal Cells
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Abstract:
The amino acid composition of oyster peptides, soy peptides, collagen peptides and perilla peptides were analyzed. Oyster peptide, soy peptide, collagen peptide, and perilla seed peptide are compounded in different proportions to affect the secretion of testosterone, dihydrotestosterone and other male hormones in mouse leydig cells (TM3) and the oligopeptide combinations were screened for improving sexual function. Tetramethylazozol blue (MTT) colorimetric method was used to determine the effect of oyster peptides, soy peptides, collagen peptides, and perilla peptides on the survival rate of TM3 cells. ELISA was used to detect testosterone, dihydrotestosterone, cyclic GMP (cGMP) content, nitrate reductase method was studied to detect nitric oxide (NO) content, WST-8 method and thiobarbituric acid method were used to detect superoxide dismutase (SOD) enzyme activity and malondialdehyde (MDA) concentration, respectively. The results showed that the four oligopeptides were rich in arginine, especially the perilla peptide, which had the highest arginine content 10.02%. Compared with the control group, oyster peptide and perilla seed peptide (both at a concentration of 400 μg/mL, volume 2:1) had the best combined effect, and the promotion effects on testosterone, dihydrotestosterone, and cGMP were 50.64%, 143.69%, and 88.44%, respectively. In addition, they can also increase the NO concentration and SOD activity of TM3 cells, and reduce the concentration of TM3 cells; maintain a stable oxidizing environment in the cells. In summary, it is shown that the combination of oyster peptide and perilla seed peptide promotes the production of male hormones by TM3 cells and has the effect of improving male sexual function.