Rapid Determination of Nitrofuran Metabolites in Procambarus clarkii by Ultrasonic Assisted Derivatization-liquid Chromatography Tandem Mass Spectrometry
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Abstract:
An ultrasonic-assisted derivatization-liquid chromatography-tandem mass spectrometry method for rapid determination of four nitrofuran metabolites in Procambarus clarkii was established. The derivatization effects of ultrasonic assisted and constant temperature oscillation were compared. The conditions of chromatography-mass spectrometry and qualitative and quantitative ion pairs were optimized, and the best experimental conditions were determined. Under the optimized experimental conditions, 0.5~50.0 ng/mL was the linear range of the four metabolites, the correlation coefficient was greater than 0.99, and the detection limit of the method was 0.5 μg/kg. The average recoveries of the four metabolites ranged from 93.3% to 104.9% and the relative standard deviation was less than 10%. The results show that the deviation of the two methods is less than 4.78%. Compared with the national standard method, this method is more simple and rapid, which can significantly shorten the sample pretreatment time and improve the detection efficiency. It has some advantages in daily detection, and can provide a basis for further development and lay the foundation for basic research.
