Optimization of β-cyclodextrin-assisted Extraction of Polyphenols from Aronia melanocarpa
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Abstract:
In this study, Aronia melanocarpa was used as the raw material, β-cyclodextrin was used as the extraction medium, and the feasibility of β-cyclodextrin-assisted extraction of polyphenols from Aronia melanocarpa was explored. On the basis of single factor experiments, the optimal concentration of β-cyclodextrin, extraction temperature and extraction time were selected for the three-factor three-level Box-Behnken response surface test, and the Design-Expert 8.0.6 software was used to obtain the optimal extraction conditions. HPCL analysis of the polyphenols obtained under the optimal extraction conditions was performed. The results showed that the optimal conditions for extracting the polyphenols in Aronia melanocarpa were: temperature, 66 ℃; extraction duration, 93 min; β-cyclodextrin concentration, 33 g/L. Under these conditions, the amount of extracted polyphenols was 12.93 mg/g. The HPLC analysis of the polyphenol extract of Aronia melanocarpa obtained by β-cyclodextrin-assisted extraction showed that the polyphenols contained in the extract were vanillic acid, epicatechin, ferulic acid, chlorogenic acid and caffeic acid, with their contents were as 239.80 μg/g, 613.00 μg/g, 17.48 μg/g, 30.93 μg/g, and 16.11 μg/g, respectively. This research shows that β-cyclodextrin can assist the extraction of polyphenols from Aronia melanocarpa, which provides a theoretical reference for the development and application of Aronia melanocarpa.
