Separation and Purification of Tetrodotoxin from the Liver of Takifugu pseudommus
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Abstract:
The separation and purification effect of chromatographic column on tetrodotoxin was studied, which provided a reference for the separation and purification process of tetrodotoxin. The raw material for this experiment was the liver of Takifugu pseudommus, TTX was extracted from the liver, and the column chromatography technology by the loading of neutral alumina column, activated carbon column, CM HF and D152 weak acid cation exchange resin column was used to determine theoptimal pH, adsorption rate and recovery rate of liquid. According to the results of the adsorption rate and purification recovery rate of the four columns, the adsorption rate and recovery rate of the D152 weakly acidic cation exchange column were 91.97% and 93.98%, respectively, and the column was effective in purifying tetrodotoxin with low loss rate. Finally, by D152 column the crude tetrodotoxin extract was separated and purified. The raw material was Takifugu pseudommus liver. The crude extract obtained from liver homogenization was purified and separated by D152 column, and then was crystallized to obtain crude tetrodotoxin with a purity of 80%. This study provides a useful reference for the separation and purification process of tetrodotoxin.
