Optimization of High-yield γ-Oryzanol Fermentation System Based on Metabolic Regulation
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
In this study, Monascuspurpureus was used to ferment Tartary buckwheat. Based on previous reseaches, the metabolic process was regulated from three aspects: cell membrane permeability, metal ion concentration and substrate induction. The fermentation system with high yield of γ-oryzanol was optimized by the response surface method. The single factor experiment results showed that the accumulation of γ-oryzanol in the fermentation product could not be promoted by ultrasonic treatment, and the addition of surfactants (Triton X-100, Tween-80, SDS), Ca2+, Mg2+, and sitosterol had a positive effect on the accumulation of γ-oryzanol. The control process parameters obtained through response surface optimization were: Triton X-100 was 3.2‰, Ca2+ was 4.2‰, ratio of ferulic acid to sitosterol (ferulicacid: sitosterol=1:1, W:W) was 0.5‰. The content of γ-oryzanol after regulation can reach 2.61 mg/mL, which was increased to 1.64 times of that before regulation, and 31.62 times higher than that of the unfermented blank group. The fermented tartary buckwheat product of Monascuspurpureus was light red with strong red yeast flavor. Furthermore, the fermentation product was rich in γ-oryzanol after metabolic regulation, which has a good industrial development prospect.
