Inhibitory Effect of Earthworm Protein Capsules on Arteriovenous Thrombosis in Rats
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Abstract:
The pharmacological activity of active earthworm protein capsules (AEPC) in animals was verified by three tests on the coagulation and blood time of mice, arterial and venous thrombosis modeling experiments and rat coagulation tests. ICR mice were randomly divided into blank control group, positive group, low-dose (22.50 mg/kg), medium-dose (45.00 mg/kg), high-dose group (90.00 mg/kg). After grouping, mice were given intragastric administration (1 mL/100 g) every day for 7 consecutive days. One hour after the last administration, blood samples were taken from the tail tip and the retrobulbar vein plexus to observe the bleeding and coagulation time. SD rats were randomly divided into 6 groups, namely sham operation group, model group, positive group (13.00 mg/kg), low-dose (15.63 mg/kg), medium-dose (31.26 mg/kg), high-dose group (62.52 mg/kg), 11 in each group. After grouping, daily intragastric administration (1 mL/100 g) was given for 7 consecutive days. One hour after the last administration, the inhibition rates of thrombosis in both common carotid arteries and inferior vena cava were calculated, and the blood samples from ocular fundus venous plexus were taken to observe prothrombin time (PT), ellagic acid activated partial thromboplastin time (APTT), thrombin time (TT), 8 animals were taken from each group. Compared with the normal group, AEPC can prolong the tail bleeding and coagulation time of mice (p<0.01), and it is dose dependent. The high-dose group (90.00 mg/kg) had the best prolonged tail bleeding activity (18.88 min) and coagulation effect (82.63 min). In addition, compared with the model group, the effect of AEPC on thrombosis was gradually increased with the increase of dose. The high-dose group (62.52 mg/kg) had the best activity (wet weight inhibition rate 76.53; dry weight inhibition rate 72.49). The experimental data show that AEPC has a significant effect on the inhibition of thrombosis.
