Antioxidant and Anti-proliferative Activities of Polyphenols from Sterculia nobilis Smith Huskon Human Lung Cancer Cells A549
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Abstract:
In this study, the free and bound polyphenols were extracted from Sterculia nobilis Smith husk using Sterculia nobilis Smith husk as the raw materials. Then, the free polyphenols were extracted with organic solvents of different polaritiesto yield a four phase-extract, namely n-hexane phase (HF), ethyl acetate phase (EAF), n-butanol phase (BF) and aqueous phase (AqF). The total phenolic contentand total flavonoid content of the free polyphenol extract, bound polyphenol extract and four-phase extract were measured. The results showed that the free polyphenolextract had higher total phenolic content and total flavonoid content than the bound polyphenol extract. Among the four phases in the four-phase extract, the EAF had the highest phenolic and flavonoid contents.DPPH free radical scavenging ability, ABTS free radical scavenging ability and reducing power assays were used to evaluate comprehensively the antioxidant activities of the bound polyphenol extract and four-phase extract. EAF showed a higher antioxidant activity, with the EC50 values for DPPH and ABTS free radicals being 2.08 mg/mL and 29.28 mg/mL. The reducing power of EAF reached 0.81 at a concentration at 10 mg/mL. Eleven phenolic compounds (epicatechin, vanillic acid, isoorientin, ferulic acid, rutin, quercitrin, myricetin, trilobatin, quercetin and kaempferol) were identified in EAF by HPLC. In addition, EAF had a dose-dependent inhibitory effect on the proliferation of human lung cancer cell A549 with an EC50 value of 85.70 μg/mL. The results indicate that the polyphenol extract from Sterculia nobilis Smith husk has significant antioxidant activity and anti-proliferative activity towards A549 cells, which is helpful for the comprehensive utilization of Sterculia nobilis Smith husk and the development of new functional agents.
