Process Optimization of Soluble Dietary Fiber from Roxburgh rose Pomace Fermented by Agrocybe aegerita and Its Evaluation on the Moistening and Laxative Function of Mice
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Abstract:
Rosa roxburghii pomace was fermented by Agrocybe aegerita to increase the content of soluble dietary fiber (SDF). The function of moistening intestines and defecation of Rosa roxburghii pomace was also investigated, which could provide the basis for the comprehensive development and utilization of dietary fiber of Rosa roxburghii pomace. Methods: on the basis of single factor and Box-Behnken experimental design, the fermentation medium was optimized to improve the yield of SDF. KM mice were randomly divided into blank control group, model control group, positive control group, SDF low (ig. 0.25 g/kg·bw), medium (ig. 0.5 g/kg·bw) and high-dose group (ig. 1 g/kg·bw). The constipation model was established with compound diphenoll. After continuous intragastric administration for 14 days, the effect of the intragastric infusion on the moistening and laxative function of mice was analyzed by measuring the propulsion rate of small intestine ink, the time of the first black stool, and the number and weight of the first black stool within 6 h. Results: the optimum preparation conditions were as follows: calcium chloride of 1%, potassium chloride of 0.6%, sugar of 4% and ratio of liquid to material of 7:1. Under these conditions, the SDF yield of Rosa roxburghii pomace was 5.86%, which was 68.78% higher than that of unfermented pomace. The results of animal experiments showed that on the basis of successful constipation model, all SDF dose groups could improve the constipation of mice, and the effect of high dose group was the most obvious. Conclusion: the preparation technology of SDF from Rosa roxburghii pomace fermented by Agrocybe aegerita was reasonable, economic and convenient, and the product could have a good effect of moistening and laxative.
