Optimization of Ultrasonic Assisted Extraction of Volatile Oil from Aucklandia odorifera and Its Chemical Components Analysis
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Abstract:
In this work, response surface methodology (RSM) was used to optimize the ultrasonic assisted extraction of volatile oil from Aucklandia odorifera. On the basis of single factor experiment, the box Behnken response surface method was used to design the experiment. The yield of Aucklandia volatile oil was used as the response index and the effects of liquid-solid ratio, extraction temperature and ultrasonic time on the yield of Aucklandia volatile oil were investigated. The volatile oil extracted by ultrasonic under the optimal extraction process was analyzed by GC-MS, and the peak area normalization method was used. Their content was also determined. The results showed that the optimum technical parameters were as follows: the ratio of material to liquid of 1:20, the extraction temperature of 50 ℃ and the ultrasonic time of 36 min. The yield of volatile oil under this condition was 5.60%, which was closeto the theoretical value of 5.70%. The results showed that this method was more accurate. GC-MS analysis indicated that a total of 35 compounds were identified, accounting for 92.03% of the total volatile oil components. The components with high contents were caryophyllin, (-)-isoaromatic hydrocarbon-(V), 1-methylcycloheptene, 1,4-cyclobutadiene, 4-(1-methylamyl)-1-cyclohexene-1-carboxylaldehyde, 11,11-dimethyl spirocyclic [2,9] dodecane-3,7-diene. The content of 1,4-cyclobutadiene was highest. Therefore, the technology of ultrasonic assisted extraction of volatile oil from Radix Aucklandiae could beaccurate and reliable, and the ultrasonic assisted method could be applied to the extraction of volatile oil from Radix Aucklandiae. GC-MS method could provide a good basis for the quality identification of Radix Aucklandiae.
