Synthesis of the Furfural Residue Composite for Immobilized Pectinase
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
In this paper, furfural residue was used as raw material for the sulfonated pretreatment. The furfural residue-chitosan-glutaraldehyde composite material and the sulfonated furfural residue-chitosan-glutaraldehyde composite material were obtained by wrapping chitosan and crosslinking with glutaraldehyde, using electrostatic self-assembly; the appearance and properties of the two new kinds of materials were characterized by FT-IR and SEM. Then, they were used for immobilizing pectinase, and the catalytic performance and stability of the novel immobilized enzyme were optimized by single factor variable method. The optimum catalytic conditions for the immobilized enzyme of uncarbonized furfural residue composite material were determined as: pH=3.5, pectinase concentration = 50 mg/mL, pectin concentration = 15 mg/mL, reaction time = 120 min, reaction temperature = 45 ℃. The optimum catalytic conditions for the immobilized enzyme of carbonized furfural residue composite material were as below: pH = 3.5, pectinase concentration = 20 mg/mL, pectin concentration = 10 mg/mL, reaction time = 60 min, reaction temperature = 50 ℃. The relative enzyme activities of the two immobilized enzymes were 95.52% and 95.03%, respectively. Under the optimal reaction conditions, the maximum enzyme loading of the two immobilized enzymes were 197.20 mg/g and 60.40 mg/g, respectively. The residual relative activity of immobilized pectinase in furfural residue composite was 81.78% after 8 times of recycling, and the immobilized pectinase activity of sulfonated furfural residue composite was still 88.98% after 32 days of storage at 4 ℃. The two kinds of immobilized enzymes show better loading enzyme capacity and storage stability, and have better economic value and application prospects.
